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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Immunol. 2016 Aug 26;197(7):2635–2645. doi: 10.4049/jimmunol.1600974

Figure 7. DC access to the splenic white pulp is required for the maintenance of Il-2-dependent Tr cells.

Figure 7

(A) Strategy to assess the ability of GPCR-inhibited DCs to rescue Il-2 activation (pSTAT5) in Tr cells from DC-depleted mice. CD11c-enriched cells were incubated for 60 minutes in RP-10 ±1 μg/ml pertussis toxin (PTx) prior to adoptive transfer. (B) Il-2 activation (pSTAT5) in Tr cells from mice receiving 2.5x106 CD11c+ cells treated as in A. Data pooled from two independent experiments with at least 2 mice per group. (C) Recovery of adoptively transferred DCs ±PTx pretreatment 20H post-transfer into congenically marked recipients. (D) Localization of adoptively transferred DCs within recipient mice as assessed by in vivo antibody labeling. 2.5x106 CD11c+ cells ±PTx pretreatment were transferred i.v. Error bars in all panels represent mean ±SEM. **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001, as calculated by one-way ANOVA with Tukey’s post hoc test