. Author manuscript; available in PMC: 2017 Oct 1.

Published in final edited form as: J Immunol. 2016 Aug 26;197(7):2864–2879. doi: 10.4049/jimmunol.1600043

Table III.

Cytokines in BAL fluid at 24 h after instillation of PBS or S. pneumoniae (SP) in WT or Nrf2 null mice

Analyte (pg/mL)	WT			Nrf2 null
Analyte (pg/mL)	PBS (n=4)	S. pneumoniae 6.88 ± 0.13 × 10⁶ CFU per mouse (n=5)	S. pneumoniae 1.38 ± 0.038 × 10⁷ CFU per mouse (n=5)	PBS (n=4)	S. pneumoniae 6.25 ± 0.22 × 10⁶ CFU per mouse (n=5)	S. pneumoniae 1.13 ± 0.022 × 10⁷ CFU per mouse (n=5)
IL-1a	2.5 ± 0.55	113 ± 19 ^*	127 ± 28 ^*	4.0 ± 2.6	127 ± 20 ^*	151 ± 22 ^*
IL-1b	9.4 ± 0.55	123 ± 16	159 ± 27 ^*	15 ± 9.0	151 ± 21	238 ± 61 ^*
IL-2	2.5 ± 0.42	3.9 ± 0.20	3.2 ± 0.22	1.5 ± 0.41	6.0 ± 2.1	3.9 ± 0.36
IL-3	1.9 ± 0.18	3.6 ± 0.30	3.9 ± 0.43	2.1 ± 0.52	4.5 ± 0.56 ^*	5.1 ± 0.81 ^*
IL-4	2.1 ± 0.01	6.2 ± 0.45	7.7 ± 0.65	2.4 ± 0.47	8.0 ± 0.87	12 ± 3.1 ^*
IL-5	1.9 ± 0.16	7.8 ± 0.78 ^*	9.3 ± 1.2 ^*	2.1 ± 0.74	8.1 ± 0.85 ^*	12 ± 2.3 ^*
IL-6	1.5 ± 0.34	420 ± 59	444 ± 39	8.3 ± 7.4	599 ± 61 ^*	697 ± 203 ^*
IL-10	3.9 ± 0.27	19 ± 1.4	24 ± 2.8	4.0 ± 1.6	22 ± 2.2	45 ± 19 ^*
IL- 12(p40)	22 ± 3.5	561 ± 55 ^*	596 ± 153 ^*	27 ± 6.3	884 ± 55 ^*	722 ± 169 ^*
IL- 12(p70)	8.3 ± 2.6	122 ± 8.7 ^*	126 ± 29 ^*	16 ± 11 (n=3)<LLOQ (n=1)	214 ± 12 ^*,^†	179 ± 32 ^*
IL-13	41 ± 3.0	58 ± 3.0	63 ± 3.1	32 ± 4.4	67 ± 6.5 ^*	76 ± 9.7 ^*
IL-17A	5.5 ± 0.39	202 ± 30	134 ± 32	6.0 ± 2.0	274 ± 87 ^*	259 ± 53 ^*
Eotaxin	1388 ± 206	1883 ± 77	1814 ± 129	1464 ± 128	1823 ± 96	1813 ± 105
G-CSF	54 ± 5.7	4652 ± 695	4609 ± 553	249 ± 199	5207 ± 1125 ^*	6486 ± 1985 ^*
GM-CSF	34 ± 1.5	94 ± 17	57 ± 3.4	36 ± 6.6	170 ± 47 ^*	71 ± 10
IFN-γ	15 ± 1.5	91 ± 20	553 ± 209	10 ± 2.8	90 ± 29	603 ± 218
KC	15 ± 3.4	160 ± 14	232 ± 27 ^*	33 ± 25	344 ± 38 ^*, ^†	277 ± 60 ^*
MCP-1	51 ± 3.0	1148 ± 146	1626 ± 470 ^*	56 ± 17	1578 ± 351 ^*	2587 ± 393 ^*
MIP-1α	12 ± 1.4	772 ± 141	1590 ± 214	75 ± 71	2210 ± 265	7051 ± 4950 (n=4), >UL (n=1)
MIP-1β	2.7 ± 0.29	180 ± 17	213 ± 38	12 ± 9.9	371 ± 43	1237 ± 766
RANTES	0.95 ± 0.15	493 ± 86	956 ± 228	11 ± 9.3 (n=3)<LLOQ (n=1)	683 ± 83	1827 ± 539 ^*
TNF	6.0 ± 0.57	4823 ± 1243 ^*	3342 ± 420	21 ± 17	8878 ± 1186 ^*, ^†	3794 ± 411 ^*

Values are mean ± SEM. The number of samples in each group (n) is shown in the column subheadings unless otherwise indicated in the individual cells. <LLOQ, below the lower limit of quantitation; >UL, exceeds upper limit of quantitation.

, significantly different compared with PBS control of the same genotype;

^†

, significantly different compared with WT that received the same treatment (p≤0.05, ANOVA and Tukey-Kramer HSD post hoc test).