. Author manuscript; available in PMC: 2017 Oct 1.

Published in final edited form as: J Immunol. 2016 Aug 26;197(7):2864–2879. doi: 10.4049/jimmunol.1600043

Table IV.

Markers of injury and DAMPs measured in BAL fluid at 24 hours after instillation of PBS or S. pneumoniae (SP) in WT or Nrf2 null mice

Analyte	WT			Nrf2 null
Analyte	PBS (n=4)	SP 6.88 ± 0.13 × 10⁶ CFU per mouse (n=5)	SP 1.38 ± 0.038 × 10⁷ CFU per mouse (n=5)	PBS (n=4)	SP 6.25 ± 0.22 × 10⁶ CFU per mouse (n=5)	SP 1.13 ± 0.022 × 10⁷ CFU per mouse (n=5)
Protein (μg/ml)	77 ± 2.5	128 ± 4.2	165 ±6.9	81 ± 15	134 ± 7.1	179 ± 36 ^*
dsDNA (ng/ml)	43 ± 9.5	573 ± 17 ^*	581 ± 63 ^*	115 ± 57	598 ± 17 ^*	512 ± 43 ^*
sICAM-1 (ng/ml)	147 ± 8.5	246 ± 16 ^*	248 ± 33 ^*	130 ± 6.0	311 ± 18 ^*	269 ± 24 ^*
S100A8 (net FL)	9.8 ± 6.8	6490 ± 535 (n=3) ^*	6264 ± 1677 ^*	1615 ± 1607	6554 ± 318 ^*	7472 ± 754 ^*
RAGE (net FL)	22 ± 1.2	114 ± 18 (n=3)	235 ± 57	18 ± 2.6	1194 ± 1061	418 ± 215
S100A9 (net FL)	552 ± 260	8575 ± 333 (n=3) ^*	8678 ± 214 ^*	2621 ± 2228	8330 ± 97 ^*	8599 ± 185 ^*

Values are mean ± SEM. The number of samples in each group (n) is shown in the column subheadings unless otherwise indicated in the individual cells. Net FL, net fluorescence.

, significantly different compared with PBS control of the same genotype (p≤0.05, ANOVA and Tukey-Kramer HSD post hoc test).