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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Immunol. 2016 Aug 26;197(7):2864–2879. doi: 10.4049/jimmunol.1600043

Table VIII.

Biological pathways and processes that are significantly enriched in WT compared to Nrf2 null lungs during S. pneumoniae pneumonia

Gene Set ID Description Numb
er of
genes
Enric
hmen
t
Score
Norm
alized
Enric
hmen
t
Score
FDR
Detoxification and antioxidant pathways
REACTOME
7099083
Glutathione conjugation 27 −0.82 −2.38 0.00
KEGG MMU00480 Glutathione metabolism 51 −0.71 −2.35 0.00
GO0004364 Glutathione transferase activity 28 −0.76 −2.29 0.00
REACTOME
7098590
Phase II conjugation 69 −0.64 −2.25 0.00
GO0016765 Transferase activity, transferring alkyl or aryl
(other than methyl) groups
55 −0.67 −2.24 0.00
KEGG MMU00982 Drug metabolism - cytochrome P450 54 −0.67 −2.23 0.00
KEGG MMU00980 Metabolism of xenobiotics by cytochrome
P450
52 −0.68 −2.27 0.00
BIOCYC
MOUSE_PWY-
4061
glutathione-mediated detoxification 22 −0.78 −2.16 0.00
KEGG MMU05204 Chemical carcinogenesis 76 −0.59 −2.14 0.00
WIKIPATHWAYS
WP1245
Keap1-Nrf2 13 −0.83 −2.02 0.02
GO0016903 Oxidoreductase activity, acting on the
aldehyde or oxo group of donors
50 −0.62 −2.06 0.03
GO0006749 Glutathione metabolic process 46 −0.66 −2.15 0.05
WIKIPATHWAYS
WP164
Glutathione metabolism 17 −0.74 −1.93 0.06
REACTOME
7099084
Sulfur amino acid metabolism 20 −0.72 −1.92 0.06
GO1900750 Oligopeptide binding 15 −0.77 −1.98 0.07
Immune cell signaling
REACTOME
7099241
Generation of second messenger molecules 22 −0.75 −2.10 0.00
GO0042101 T cell receptor complex 13 −0.83 −2.05 0.04
GO0001772 Immunological synapse 31 −0.66 −1.98 0.06
KEGG MMU05340 Primary immunodeficiency 35 −0.61 −1.89 0.09
Olfactory processes
KEGG_MMU0474
0
Olfactory transduction 1057 −0.41 −1.98 0.03
GO0004984 Olfactory receptor activity 1083 −0.42 −2.01 0.06
GO0005549 Odorant binding 453 −0.44 −1.99 0.06

GSEA was used to identify gene sets that show statistically significant, concordant differences in expression between WT and Nrf2 null lungs during S. pneumoniae pneumonia (FDR < 0.1). . The table lists the biological pathways or processes showing enrichment in WT compared to Nrf2 null lungs during pneumonia, sorted by functional categories. The genes that contributed to core enrichment are listed in Supplemental Table II.