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. 2016 Sep 17;16:366. doi: 10.1186/s12906-016-1344-1

Table 1.

Stock solution for assay

Stock solution concentration (mM) Volume (μl) Final concentration (mM)
K-PO4 buffer, pH 6.8 250 4760.0 100
MgCl2 60 39.7 0.2
ATP 60 39.7 0.2
Inhibitor 12 991.7 1
KCl 100 1190.0 10
Shikimic acid 100 238.0 2
Enzyme/dH20(nM) 100 1785.0 15
nH2O 2856.0