Table 3.

Gradient Table for Gradient Pump

Time (min)	Flow (mL/min)	% HOH	% MeOH	% 0.1 % FA HOH	% CAN
0.0	5.5	0	65	35	0
2.0	5.5	0	65	35	0
15.0	5.5	0	75	12	13
25.0	5.5	0	0	0	100
40.0	5.5	0	0	0	100
44.0	5.5	0	65	35	0
50.0	5.5	0	65	35	0

Column heater temperature: 60 °C

PDA: Scanning 210–600 nm

Collection: Hold time: 1 min, 2 min collection intervals for 20 vials