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. 2016 Sep 17;9:506. doi: 10.1186/s13071-016-1790-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Specific expression of Ha24 in Hyalomma asiaticum. a Analysis of specific expression of Ha24 gene according to the sex. Lanes 1–4, 5–8: the cDNA of unfed male adult ticks, unfed female adult ticks, fed male adult ticks and partially fed female adult ticks (Lanes 1–4: actin primers; Lanes 5–8: Ha24 gene specific primers). b Expression of Ha24 in different development periods. Lanes 1–3, 4–6: the cDNA of eggs, larvae, and nymphs (Lanes 1–3: actin primers; Lanes 4–6: Ha24 gene specific primers). c Expression of Ha24 in the salivary glands and midguts of H. asiaticum. Lanes 1–2, salivary glands and midguts with actin primers, respectively; Lanes 3–4, salivary glands and midguts with Ha24 gene specific primers, respectively