Figure 3. TRIBE demonstrates Hrp48 preferentially binds the 3’ UTR of transcripts.
A) Both CLIP signal and editing sites are enriched in the 3'UTR. Metagene quantification of the location of either CLIP peaks or TRIBE edits. Background indicates the proportion of the fly transcriptome composed of the indicated regions. The majority of TRIBE editing sites are near CLIP peaks (B). The fraction of editing sites within a certain distance of a CLIP peak was quantified for both endogenous Hrp48 and Hrp48-TRIBE. 0 indicates that the editing site was within the bounds of a CLIP peak. C) TRIBE targets are a subset of CLIP targets. Venn diagram overlap of genes between all expressed genes, all TRIBE target genes and all genes with at least one statistically significant CLIP peak. D) TRIBE targets are more CLIP enriched. Frequency distribution of per gene CLIP enrichments of all CLIP target genes and TRIBE genes that have CLIP signal. The overlap between the top 25% ranked CLIP targets (highlighted in pink) and TRIBE targets is inset.