Sequential treatment of siAkt and PTX against MKN28 cells. (A) The index for sequential treatment of siAkt and PTX; DMSO treatment as a control (NT, ●), siAkt transfection (siAkt only, ○), simultaneous treatment of siAkt and PTX (siAkt & PTX, ▼), siAkt transfection after PTX treatment in sequential manner (PTX→siAkt, △), and PTX treatment after siAkt transfection in sequential manner (siAkt→PTX, ■). (B) Cellular microscopic images for MKN28 cells after the sequential treatment of siAkt and PTX at the indicated treatment time; △t = 12, 24, and 48 h. (C) Normalized proliferation profiles of MKN28 cells after the sequential treatment of siAkt and PTX. (D) ΔNormalized cell index calculated from (C). Non-treatment condition was used as a control. *p<0.01.