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. 2016 Sep 7;13(9):708–716. doi: 10.7150/ijms.15501

Figure 5.

Figure 5

Apoptosis-related gene expressions after the sequential treatment of siAkt and PTX on gastric cancer cell lines. (A) The index for sequential treatment of siAkt and PTX; DMSO treatment as a control (NT, ●), siAkt transfection (siAkt only, ○), simultaneous treatment of siAkt and PTX (siAkt & PTX, ▼), siAkt transfection after PTX treatment in sequential manner (PTX→siAkt, △), and PTX treatment after siAkt transfection in sequential manner (siAkt→PTX, ■). Total RNA was extracted from the cells at 24 h of treatment time. The RNA samples were subjected to qRT-PCR to analyze apoptosis-associated genes (Akt, Bcl-xL, Bcl-2, Bad, and Caspase-3) of interest in (B) MKN28 and (C) MKN45 cells. *p<0.01.