Figure 3.

CD36 protein is increased in H9c2 cells with bone morphogenetic protein receptor type 2 mutation. (A) CD36 protein (red) is localized in cell membrane and cytoplasm of control (C), mutant1 (M1), and mutant2 (M2) cells with greater staining in both mutant lines. Nucleus is stained blue. (B) Total CD36 protein is increased in M1 (n = 4; *P < 0.05) but not M2 (n = 6) compared with C cells (n = 6). Bar graph represents total protein. (C) In plasma membrane (PM) fraction, CD36 is increased in M2 cells (n = 4; *P < 0.05) but not in M1 cells (n = 4) compared with C cells (n = 4); in cytoplasmic fraction, CD36 was similar between C (n = 4), M1 (n = 4), and M2 (n = 4) cells. (D) ¹⁴C palmitate uptake is observed in M2 cells (n = 3; P < 0.05) but not M1 (n = 3) and C (n = 3) cells. HSP70 = heat shock protein 70.