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. 2016 Sep 12;7:12525. doi: 10.1038/ncomms12525

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Copyright © 2016, The Author(s)

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(a) Protein abundance of MIEL1 and MYB96 in the presence of ABA. Seeds were germinated on MS medium supplemented with or without 1 μM ABA and incubated for the indicated time periods after cold stratification. The molecular weight (kDa) was indicated on the right side of the gel. (b) Effects of MG132 on ABA-dependent MIEL1 degradation. Stratified seeds were treated with 1 μM ABA and/or 50 μM MG132 for 24 h. (c) Changes in ubiquitination of MYB96 in the presence of ABA. Stratified seeds were treated with 1 μM ABA for 24 h and used for the total protein extraction. (d) Effects of ABA on MYB96 stability in miel1-1. Seeds were germinated on MS medium supplemented with or without 1 μM ABA and incubated for the indicated time periods after cold stratification. DAC, days after cold stratification; IP, immunoprecipitation; Ub, ubiquitinated MYB96 proteins; WB, Western blot analysis.