Table 2. The binding affinity of 3I14 germline variants.
| 3I14 |
H5-VN04 |
H3-PE09 |
H1-CA09 |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Variants | Kd (nM) | Kon (M−1 s−1) | Koff (s−1) | Kd (nM) | Kon (M−1 s−1) | Koff (s−1) | Kd (nM) | Kon (M−1 s−1) | Koff (s−1) |
| GL | n* | n* | n* | 4.02 | 1.39E+05 | 5.56E−04 | 0.0597 | 1.09E+05 | 6.50E−06 |
| (−Δ15.3) | (−Δ13.9) | (Δ4.7) | (Δ7.5) | ||||||
| mHgL | 7.71 | 2.58E+05 | 1.99E−03 | 0.658 | 1.92E+05 | 1.26E−04 | <0.001† | 1.38E+05 | <1.0E−07† |
| (−Δ7.5) | (−Δ5.2) | (−Δ2.5) | (−Δ3.2) | ||||||
| gHmL | 1.95 | 4.34E+05 | 8.44E−04 | 0.733 | 1.71E+05 | 1.25E−04 | <0.001† | 1.55E+05 | <1.0E−07† |
| (−Δ1.9) | (−Δ2.2) | (−Δ2.7) | (−Δ3.1) | ||||||
| 3I14 WT | 1.02 | 3.75E+05 | 3.83E−04 | 0.263 | 1.52E+05 | 3.99E−05 | 0.279 | 1.74E+05 | 4.87E−05 |
WT, wild type.
n* indicates no binding detected.
†indicates no detectable dissociation.
(Δ) indicates the fold increase or (−Δ) fold decrease compared with WT.