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. 2016 Sep 5;4(17):e12902. doi: 10.14814/phy2.12902

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Increase in miR‐148b in muscle cell alters insulin signaling. Human primary myotubes were transfected with anti‐miR‐148b (miRCURY‐148b) or anti‐miRcontrol (miRCURY‐CTRL), and with a plasmid expressing pre‐miR148b (pmiR‐148b) or a control plasmid (pmiR‐CTRL). Phosphorylation of PKB and MAPK in response to 10 nmol/L and 100 nmol/L insulin was quantified by Western blotting. Results are presented as fold changes in pPKB/PKB ratios after miR‐148b inhibition (A) or overexpression (B), and as pMAPK/MAPK ratios after miR‐148b inhibition (C) or overexpression (D). Values are means ± SEM for n = 6 experiments. *indicates P < 0.05 compared to control conditions.