Figure 2. Addition of thiourea or 2,2′-dipyridyl causes a possibly non-specific increase in survival upon ObgE* expression.

Exponential-phase cultures of E. coli pBAD33, E. coli pBAD33-obgE or E. coli pBAD33-obgE* were induced with 0.2% arabinose for 2 hours and thiourea or 2,2′-dipyridyl was simultaneously added to the culture. (a) Addition of thiourea or 2,2′-dipyridyl increases survival in the presence of ObgE*. (b) The expression level of ObgE* was determined by using a fluorescent fusion of ObgE* with Venus. ObgE*-Venus expression is lowered in the presence of 2,2′-dipyridyl. Error bars represent the standard error of the mean, n = 3. Student’s t test: ^#p-value < 0.05, ^##p-value < 0.01, ^###p-value < 0.001 in comparison with ‘No scavengers’.