Figure 3. Nox2-derived ROS contributes to the differentiation of miPSCs into miPSC-ECs.

(A) Exogenous H₂O₂ (10 μM) was administered at day 2, 3 and 4, and quantitative PCR analyses showed the increase of endothelial markers CD31, CD144, VEGF and Ang-1 mRNA levels in WT miPSC at day 5 of differentiation in response to H₂O₂ stimulation (n = 3; *P < 0.05 vs. control). (B,C) Nox2 overexpression using Ad-Nox2 in WT miPSCs during EC differentiation at day 2, and cells were treated with or without DPI (1 μM) at day 4 for 48 hours. Quantitative PCR analyses showing the increase of CD31 and CD144 (B), as well as VEGF and Ang-1 (C) mRNA levels induced by Nox2 overexpression was suppressed by DPI. (n = 3; *P < 0.05, **P < 0.01).