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. 2016 Sep 19;6:33709. doi: 10.1038/srep33709

Figure 2. Rd inhibited the migration of 4T1 cells.

Figure 2

4T1 cells were seeded in triplicate into Essen ImageLock 96-well plates in the number of 20,000 cells per well. Cells were then serum-starved for 6 h and confluent cell layers were scratched using Essen Wound Maker to generate wounds approximately 600 μm wide, which was followed by treatment with vehicle or Rd at indicated concentrations including 50, 100 and 150 μM, respectively. Images were automatically acquired and registered by the IncuCyte ZOOM imaging system using time-lapse bright field microscopy. Typical kinetic updates were recorded at 2 h intervals for 48 h. (A) Representative images of wound mask of 4T1 cells treated with Rd in scratch/wound migration assay. The initial wound mask (in blue) and wound closure (in yellow) were recorded by IncuCyte. (B) RWD was generated to quantify 4T1 cell migration. *Compared to vehicle control, p < 0.05.