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. 2016 Sep 19;6:33709. doi: 10.1038/srep33709

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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miR-18a expression in the absence or presence of Rd treatment was analyzed using real-time PCR in cultured 4T1 cells (A) and 4T1 cell-derived tumors (B). Real-time PCR was also performed to analyze the relative mRNA expression of Smad2 in vehicle or Rd-treated 4T1 cells (C) and in 4T1-derived tumors after vehicle or Rd treatment (D). Relative fold change in the expression level was plotted against that of vehicle control. *Compared to that from the vehicle control, p < 0.05.