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. 2016 Sep 19;6:33709. doi: 10.1038/srep33709

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Copyright © 2016, The Author(s)

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Total proteins from homogenized 4T1 cell-derived tumors were subjected to Western blot analyses to examine the protein levels of TGFβ1, TGFβR1 and Smads after vehicle or Rd treatment in BALB/c mice. Signals were visualized and recorded by UVP BioSpectrum imaging system (A). The quantification of the protein levels of TGFβ1 (B), TGFβR1 (C), Smad2 (D), Smad3 (E) and Smad4 (F) was performed by Launch VisionWorks LS. β-actin was used as an internal control for normalization. Relative fold change in the expression level was plotted against that of vehicle control. *Compared to that from the vehicle control, p < 0.05.