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. 2016 Aug 29;113(37):E5444–E5453. doi: 10.1073/pnas.1604256113

Fig. 3.

Fig. 3.

CD122 identifies exhausted cells in individuals infected with HCV. PBMCs were collected during the first year of HCV infection from individuals who had high viral titers and developed persistent viremia as described in Fig. S3A. (A) Expression of extracellular PD-1 and intracellular Eomes in CD122+ (Upper Right) and CD122− (Lower Right) HCV-specific CD8+ T cells. Gates for CD122+ and CD122− cells are set based on fluorescence minus one (FMO) controls. Values indicate the frequency of cells in each quadrant. (B) Frequency of CD122− (filled circles) and CD122+ (open circles) HCV-specific CD8+ T cells positive for the indicated molecules. (C) Frequency of CD122− (filled circles) and CD122+ (open circles) CD45RA−CCR7− effector memory CD8+ T cells positive for the indicated molecules. (D) Expression of PD-1 and Eomes in CD122− (filled circles) and CD122+ (open circles) CD45RA−CCR7− effector memory CD8+ T cells represented by their MFI. *P < 0.05; **P < 0.005; NS P ≥ 0.05; two-tailed unpaired Student’s t test. Data are pooled from five to eight distinct time points from two to three infected patients processed independently.