Table 1.
Category | Result |
Data collection | |
No. of overlapping segments | 146,696 |
No. of unique molecules | ∼300,000 |
Pixel size (Å) | 1.08 |
Defocus range (µm) | 0.5–3.5 |
Voltage (kV) | 300 |
Electron dose (e− Å−2) | 20 |
Refinement | |
Helical symmetry | 106.65°/5.45 Å |
Resolution (Å) | 4.0 |
Free shell (Å) | 4.3–4.0 |
FSCavg (work)/FSCavg (free) | 0.81/0.45 |
Cwork/Cfree | 0.81/0.43 |
Rwork/Rfree | 31.8/48.5 |
Deviations from idealized geometry | |
Bond lengths (Å) | 0.0018 |
Bond angles (°) | 0.47 |
Model quality | |
Molprobity score | 2.15 (100th percentile) |
Clash score, all atoms | 7.38 (100th percentile) |
Good rotamers (%) | 91.7 |
EMRinger score | 3.42 |
Ramachandran statistics | |
Favored (%) | 96.2 |
Outlier (%) | 1.7 |