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. 2016 Aug 25;113(37):10364–10369. doi: 10.1073/pnas.1611038113

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Fig. 4. — Live-cell NL-SIM based on patterned photoactivation. (A and B) PA NL-SIM images of frame 1 and frame 25 from Movie S1 of a COS-7 cell transfected with Skylan-NS–Lifeact at 100 W/cm² illumination intensity. (C) Zoomed-in view of the boxed region in A. (D and E) PA NL-SIM images of frame 1 and frame 8 from Movie S2 of a COS-7 cell transfected with Dronpa–Lifeact at 250 W/cm² illumination intensity. (F) Zoomed-in view of the boxed region in D. (G and H) PA NL-SIM images of frame 1 and frame 60 from Movie S3 of a COS-7 cell transfected with rsEGFP2–Lifeact at 50 W/cm² illumination intensity. (I) Zoomed-in view of the boxed region in G. [Scale bars, 5 μm (B, E, and H) and 1 μm (C, F, and I).] Gamma value, 0.5 for all images.