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Comparison between Skylan-NS and Skylan-S. (A, Upper) Sequence alignment of Dronpa, mEos3.1, Skylan-S, and Skylan-NS. Mutation from mEos3.1 to Skylan-NS is marked by a red frame. (Lower) Photoswitching kinetics of Skylan-NS and Skylan-S. E. coli cells expressing (B) Skylan-NS and (C) Skylan-S were continuously excited with 488 nm TIRF illumination (0.24 mW), and pulsed 0.01-s bursts of illumination with 405-nm light at 0.2 mW were used to photoactivate the fluorescent proteins.
