Skip to main content
. 2016 Aug 25;113(37):10418–10423. doi: 10.1073/pnas.1521363113

Fig. 3.

Fig. 3.

HDA6 regulates BR signaling through BIN2 and its homologs. (A–C) Hypocotyl length of Col-0 and the BRI1-GFP line (A), Ws-2 and bin2-3 bil1 bil2 plants (B), and En-2 and bes1-D plants (C). Plants were grown in the dark on medium containing different concentrations of TSA. (D) HDA6 partially rescued the dwarf phenotype of bri1-301. (E) HDA6 can partially suppress the dwarf phenotype of bin2-1. (F) Expression levels of the BR-responsive genes CPD and DWF4 in bri1-301, axe1-5 bri1-301, and HDA6-YFP bri1-301. Their expression in bri1-301 was defined as 1. (G) Expression levels of the BR-responsive genes CPD and DWF4 in bin2-1, axe1-5 bin 2-1, and HDA6-YFP bin 2-1. Their expression in bin-2-1 was defined as 1. (H) HDA6-RNAi cannot suppress the seedling phenotype of the bin2-3 bil1 bil2 triple mutant. (I) Expression levels of CPD, DWF4, and HDA6 in bin2-3 bil1 bil2 and HDA6-RNAi bin2-3 bil1 bil2 plants. Their expression level in Ws-2 was defined as 1. (J) HDA6-RNAi hardly affects hypocotyl elongation in the bin2-3 bil1 bil2 background growing on medium containing 1 μM BRZ220 in the dark. Error bars represent SE. *P < 0.05, **P < 0.01, and ***P < 0.001.