Fig. S7.

Confirmation of the phenotype of missing Lgr5⁺ stem cells by high sensitive single-molecule FISH. Shown are high sensitive two-color single molecule FISH using Lgr5 (green) and Reg4 (red) probes. Apical actin network was stained by phalloidin (white). Lgr5 is expressed between Reg4-expressing cells at crypt bottoms of Reg4^+/+ upon 6 d of DT injection (A and B = 4 mice). Both Lgr5 and Reg4 expressions were not detected in Reg4^DTR-Red/+ upon 6 d of DT administration (B). Asterisks indicate nonspecific signals. (Scale bars: 20 µm.)