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Confirmation of Reg4 expression at both mRNA and Protein level. Time course of Reg4 mRNA expression in Reg4DTR-Red/+ organoid that was treated by DT and PBS at 0, 1, 2, 3, 4, 5, and 6 d after DT administration. (A) qRT-PCR analysis represented mRNA expression of Reg4 in Reg4DTR-Red/+ organoids cultured with PBS (red) and DT (blue). (B) Western blot analysis of Reg4 and GAPDH was used as a loading control.
