Fig. 1.

Schematic representation of the graphical topology-prediction algorithm TopGraph. (A) Cylinders represent sequence segments (nodes in the graph) in either insertion orientation. Curved gray arrows represent edges connecting nodes; curved black arrows denote the minimum-energy path. Faded cylinders represent nodes not in the minimum-energy path. (B) A constraint (dashed lines) eliminates all edges bypassing it, guaranteeing, by construction, that only segments satisfying the constraint are chosen. Insets show final predicted topology.