Table S7.

Occurrence of TGCT-affected males per generation in A1cf^KO/+ and Ago2^KO/+ reciprocal backcrosses

Crosses	Offspring genotype	No. generation	No. males	Affected males		Test score χ2, P-value
				No.	Frequency, %
A1cfKO/+ test crosses
A1cfKO/+ × 129/Sv	Wild-type	1	28	0	0	1.4y, NS
		2	81	1	1.2
		3	17	0	0
	A1cfKO/+	1	32	2	6.2	0.4y, NS
		2	89	2	2.2
		3	17	0	0
129/Sv × A1cfKO/+	Wild-type	1	10	0	0	0.6y, NS
		2	44	1	2.3
		3	53	4	7.5
	A1cfKO/+	1	22	1	4.5	0.7y, NS
		2	52	2	3.8
		3	62	0	0
Ago2KO/+ test crosses
Ago2KO/+ × 129/Sv	Wild-type	1	46	0	0	1.5y, NS
		2	45	3	6.7
		3	24	2	8.3
	Ago2KO/+	1	27	0	0	0.5y, NS
		2	44	0	0
		3	20	1	5
129/Sv × Ago2KO/+	Wild-type	1	51	2	3.9	0.1y, NS
		2	93	4	4.3
	Ago2KO/+	1	50	1	2	<0.1y, NS
		2	103	4	3.9

The χ² contingency tests were used to compare occurrence of TGCT-affected males in each generation. χ² and P values are indicated for each test result (df = 2). NS indicates results that did not pass the threshold of statistical significance. y indicates Yate’s correction was applied to the test.