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All NIS mutants are properly targeted to the plasma membrane. Nontransfected HEK cells and HEK cells transfected with WT NIS or the indicated NIS mutants [(A) S66, (B) Q194, (C) Q263] were biotinylated under nonpermeabilized conditions and immunoblotted with anti-NIS Abs (Upper) or anti-Na+/K+ ATPase Abs, as a loading control (Lower).
