Fig. 2.

Combination treatment of vitamin C and 5-aza-CdR up-regulates viral-defense genes. (A) Comparison of the expression of IFN-responsive genes upon combination treatment vs. 5-aza-CdR treatment in HCT116, HL60, and SNU398 cells. Treatment was as described in Fig. 1A. Transcripts were analyzed by microarray at 5 d after treatment. Orange indicates genes up-regulated in at least two cell lines (genes are listed at the right); gray denotes genes up-regulated in only one line. (B) A log2 plot of the expression of dsRNA defense genes after treatment with vitamin C, 5-aza-CdR, or their combination vs. untreated HCT116 cells. Values are calculated from fragments per kilobase per million fragments mapped and are averaged from two independent RNA-seq datasets.