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. 2016 Aug 29;113(37):10238–10244. doi: 10.1073/pnas.1612262113

Fig. 5.

Fig. 5.

DHA elicits effects similar to those of vitamin C, but other antioxidants do not. (A) Dot blot analysis of global 5hmC levels with an antibody against 5hmC in HCT116 cells untreated or treated with 300 nM 5-aza-CdR for 24 h, daily doses of vitamin C or DHA at 57 μM, daily doses of GSH at 4.9 mM or DTT at 500 μM, and the combination of 5-aza-CdR with vitamin C, DHA, GSH, or DTT. (B and C) Expression levels of HERV-Fc1 (B) and LTR12C (C) by quantitative RT-PCR in HCT116 cells on day 5 after treatment. Values are presented as mean ± SD of three independent experiments.