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. 2016 Jun 1;44(16):7691–7699. doi: 10.1093/nar/gkw488

Figure 1.

Figure 1.

(A) Gel retardation assay demonstrating the interaction between ssDNA oligonucleotide and increasing quantity of his-tagged forms of RecA(wt), RecA-G288Y, RecA-Q300A, RecA-N304D and non-tagged RecA(wt). 0.05μM ssDNA (60mer) was incubated with 0; 0.25; 0.5; 1 and 2 μM RecA-WT or RecA allele. RecA interaction with oligonucleotide is affected neither by the specific recA mutations nor by the presence of polyhistidine tag. (B) Kinetic of strand exchange (D-loop formation) promoted by his-tagged forms of RecA(wt), RecA-G288Y, RecA-Q300A, RecA-N304D and non-tagged RecA(wt). The D-loop formation was analyzed 0, 5, 10, 20 and 40 min after initiation of dsDNA uptake into recA filament. The graph represents the average and standard deviation of three independent experiments.