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. 2016 Jun 1;44(16):7691–7699. doi: 10.1093/nar/gkw488

Figure 2.

Figure 2.

Expression of RecA (A) cleavage of UmuD (B) and of LexA (C) in vivo. All cell extracts are made from cultures grown under standard conditions without genotoxic stress. (A) The level of expression of RecA in recA-Q300A and N304D extracts is intermediate between wt recA+ and lexA(Def) strains, illustrating partial SOS induction in Q300A and N304D strains. No induction is seen in allele G288Y. A similar conclusion can be reached from experiments monitoring UmuD cleavage into UmuD’ (B) or the disappearance of the intact form of LexA (C). All three assays suggest a relatively modest SOS induction in strain Q300A and a more robust one in strain N304D. oc-umuD'C is a strain that expresses chromosomal umuD'C under the control of a constitutive promoter.