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. 2016 Jun 2;44(16):7722–7741. doi: 10.1093/nar/gkw506

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 10. — Mutation of the Pch2 ATP-binding site impairs the stability of the hexameric complex. (A) Whole cell extracts (WCE) prepared after 16 h in meiosis were immunoprecipitated with anti-HA antibodies. WCE and immunoprecipitates (IP) were analyzed by Western blot with both anti-HA and anti-Myc antibodies, as indicated. Strains are: DP1325 (PCH2-HA/PCH2-Myc; lane 1), DP1329 (PCH2/PCH2-Myc; lane 2) and DP1337 (pch2-K320A-HA/pch2-K320A-Myc; lane 3). To discard a problem with detection levels, in lane 2 (HA-untagged control) and lane 3, three more times of IP compared to lane 1 were loaded. (B) Schematic interpretation of the result described in (A). K and A represent a lysine and an alanine, respectively, at position 320 of Pch2.