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Impact of the PP4 phosphatase on Pch2-dependent meiotic checkpoint. (A) Time course of meiotic nuclear divisions; the percentage of cells containing two or more nuclei is represented. (B) Western blot analysis of Hop1T318 phosphorylation and Mek1 activity at the indicated time points in meiosis. PGK was used as a loading control. Strains are: DP421 (wild type), DP1247 (pph3), DP422 (zip1), DP1249 (zip1 pph3), DP1029 (zip1 pch2) and DP1245 (zip1 pch2 pph3).
