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. 2016 Jul 8;44(16):7755–7765. doi: 10.1093/nar/gkw622

Figure 1.

Figure 1.

Ku70 interacts with cyclin B1. (A) Glutathione Sepharose beads bound to GST or GST-cyclin B1 (expressed in insect cells) were incubated with bacterially expressed and purified Ku70 (lanes 1 and 2), insect cell extract containing over-expressed Ku70 (lanes 3 and 4) or HEK293 cell extract (lanes 5 and 6). The pulled-down complexes and the supernatant were analyzed by immunoblotting (IB) with antibodies against Ku70 (Interaction and Input) and GST (Input). (B) GST or GST-Ku70 were expressed in HeLa cells and pulled down from the cell-extract with glutathione beads. The presence or absence of cyclin B1 in the extract (lanes 1 and 2) and the pulled-down complexes (lanes 3 and 4) was tested by anti-cyclin B1 immunoblotting. The expression of GST and GST-Ku70 and their presence in the beads were also checked by anti-GST immunoblotting.