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The ubi3ΔN mutation leads to a deficit in 40S r-subunits. Strains TLY56.D3 (UBI3-HA) (A), TLY63.A3 (ubi3ΔN-HA) (B) and TLY12.1A (ubi3Δ) (C) were grown in YPD at 22°C to an OD600 of 0.8. Cell extracts were prepared and 10 A260 units of each extract were resolved in 7–50% sucrose gradients. The A254 was continuously measured. Sedimentation is from left to right. The peaks of free 40S and 60S r-subunits, 80S free couples/monosomes and polysomes are indicated.
