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. 2016 Jul 15;44(16):7777–7791. doi: 10.1093/nar/gkw641

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — S31ΔN protein is incorporated into translating polyribosomes. Strains TLY56.D3 (UBI3-HA) (A) and TLY63.A3 (ubi3ΔN-HA) (B) were grown in YPD medium at 30°C to mid-log phase. Cell extracts were prepared and 10 A₂₆₀ units of each extract were resolved in 7–50% sucrose gradients and fractionated. Proteins were extracted from each fraction and equal volumes analyzed by western blotting using anti-HA, anti-S8 and anti-L5 antibodies. The position of free 40S and 60S r-subunits, 80S vacant ribosomes/monosomes and polysomes, obtained from the recorded A₂₅₄ profiles, are shown. T stands for aliquots of the applied total cell extracts.