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. 2016 Jul 15;44(16):7777–7791. doi: 10.1093/nar/gkw641

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — S31 assembles in the nucle(ol)us. Localization of S31-yEGFP and S31ΔN-yEGFP upon depletion of the 40S r-subunit assembly factor Emg1. A GAL::EMG1 strain was transformed with centromeric plasmids expressing L25-eGFP, S2-eGFP, S10A-yEGFP, Ubi3-yEGFP and Ubi3ΔN-yEGFP from their respective cognate promoters. Transformants were grown in SGal-Leu medium (Gal) or shifted overnight to SD-Leu medium (Glc) to fully deplete Emg1. Hoechst was used to stain chromatin DNA. The GFP and Hoechst staining signals were simultaneously inspected by fluorescence microscopy. Arrows point to nuclear fluorescence. Approximately 200 cells were examined for each reporter, and practically all cells gave the same results as the selected cells shown in the pictures.