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The ubi3ΔN allele confers hypersensitivity to aminoglycoside antibiotics. Haploid ubi3::HIS3MX6 cells (ubi3Δ) containing the YCplac111-UBI3-HA, YCplac111-ubi3ΔN-HA, YEplac181-UBI3-HA or YEplac181-ubi3ΔN-HA plasmids were grown in SD-Leu medium and spotted in 5-fold serial dilution steps onto YPD plates without antibiotics or containing 1 mg/ml paramomycin or 5 mg/ml neomycin. Plates were incubated at 30°C for 3–5 days.
