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. 2016 Jul 25;44(16):7974–7988. doi: 10.1093/nar/gkw656

Figure 2.

Figure 2.

Binding of AsiA and MotA alters the position of σ residue 581 relative to the DNA. (A) Denaturing polyacrylamide gel showing DNA products generated by σD581C-FeBABE cleavage of 32P 5′-end labeled template strand of PuvsX DNA. Lane 1 is the G + A marker ladder; lanes 2–6 are reactions containing the indicated reagents. Orange boxes indicate the cleavage products generated in the presence of H2O2 and sodium ascorbate (NaAsc). (B) Sequence of PuvsX DNA from -46 to -7, showing the positions of the σD581C-FeBABE generated cut sites on the template strand DNA for basal or activated transcription. The −10 element and the −35 element or the MotA box are boxed.