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. 2016 Jul 27;44(16):7866–7883. doi: 10.1093/nar/gkw661

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Integration opportunity. (A) Binding of Ssb to different DNA target in presence of unlabelled RE–LE junction. Increasing concentrations of Ssb are used in the binding reaction with ss (i); 3′ overhang (ii); gap65 (iii); gap35 (iv) and gap24 (v) targets respectively. (B) Integration of cold RE–LE junction into different targets. TnpA_IS₆₀₈ (1 μM) was added after 15 min of preincubation with Ssb (i); 3′ overhang (ii); gap65 (iii); gap35 (iv) and gap24 (v) targets with increasing concentrations of Ssb respectively. (C) Comparison of target cleavage and integration efficiencies in absence and in presence of Ssb. Effect of 2.5 μM Ssb on target cleavage (i) and integration (ii).