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. 2016 Aug 2;44(16):7997–8010. doi: 10.1093/nar/gkw685

Figure 4.

Figure 4.

Homology arm length alters the frequency of co-incident insertion. (A) Flow cytometry analysis using Lef1-GFP as the donor DNA for detection and Rosa26-Neo as the donor DNA for selection. Percentage of GFP+ cells was determined for all transfected cells (top) and for cells following G418 selection (bottom). As indicated at the top of each column, homology arm length was varied for the Rosa26-Neo donor DNA and kept constant at 208/202bp for Lef1-GFP. Insertion percentage is measured by flow cytometry after gating on a transfected, GFP-negative control population. Representative microscopy images are shown in Supplementary Figure S4A. (B) Similar to (A), except homology arm length was varied for Lef1-GFP and kept constant for Rosa26-Neo at 516/495 bp. Insertion percentage is measured by flow cytometry after gating on a transfected, GFP-negative control population. Representative microscopy images are shown in Supplementary Figure S4B.