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. 2016 Sep 19;6:33561. doi: 10.1038/srep33561

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Copyright © 2016, The Author(s)

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High frequency PFs stimulation caused depolarization (ab) and calcium increase in PNs (aa, left panel; ba, left panel); for each representative cell showed, the average ΔF/F signals was obtained at the peak of the response under the different experimental conditions (pseudocolor) and an it was superimposed on the image of the PN at resting state (grayscale). While TTA-P2 bath application strongly affected the calcium transient recorded in control condition (aa, right panel), the antagonist showed no effect in presence of SR95531 (ba, right panel). Quantified relative change in fluorescence (ΔF/F) showed a large TTA-P2 sensitive component revealing that calcium influx is mostly mediated by Ca_V3 activation but only in control condition (ac, mean ± SEM, N = 5, ANOVA P < 0.001). Ca_V3-mediated calcium influx is lost when inhibition was blocked by bath application of SR95531 (bb, mean ± SEM, N = 5, ANOVA P < 0.001). High frequency PFs stimulation in low internal chloride induced MLI_dep-LTP (c, mean ± SEM, N = 6, RM ANOVA P < 0.001); PPRs (mean ± SEM) value for the baseline (t = 10 min) and the post-induction phase (t = 65 min) are shown in the panel c inset. The effect of different intracellular chloride concentrations on the PFs-induced calcium transient was evaluated in PNs from Ca_V3.1 KO mice (d). The TTA-P2 sensitive component of the calcium transient observed in low chloride (Low chloride: control = 1 ± 0.23, mean ± SEM, N = 5; TTA-P2: 0.42 ± 0.13, mean ± SEM, N = 5, P = 0.017, paired t-test) was lost when the normal chloride internal solution was used (Normal Chloride, control: 1 ± 0.23, mean ± SEM, N = 5; TTA-P2: 0.79 ± 0.08, mean ± SEM, N = 5, P = 0.446, paired t-test). Before cell averaging, the maximal ΔF/F value obtained before and after TTA-P2 bath application for each cell was normalized to the mean ΔF/F value obtained in control condition. *statistically significant difference, paired t-test. ^#statistically significant difference, t-test.