Table 2. Overview of the C. pasteurianum methylome and RM systems.
| Methylation motif (5′-3′)a | Methylation typeb | Number of methylated sites detected (percentage) | Corresponding RM activity | Putative annotation | Reference |
|---|---|---|---|---|---|
| GATC | m6A | 5,105/13,630 (37.4%) | CpaI (Type II) | R: CP6013_0098 | 18 |
| M: CP6013_0095/ | |||||
| CP6013_0096/ | |||||
| CP6013_0097 | |||||
| AAGNNNNNCTCC | m6A | 487/524 (92.9%) | CpaAII (Type I) | R: CP6013_0336 | 32 |
| M: CP6013_0337 | |||||
| S: CP6013_0338 | |||||
| CAAAAAR | m6A | 945/3,945 (24.0%) | NI | M: CP6013_1459 | This study21 |
| GRTAAAG | m6A | 987/2,612 (37.8%) | NI | RM: CP6013_0727 | This study21 |
| Inactive | Inactive | Inactive | NI | M: CP6013_0738 | 21 |
| CGCG | m5C | ND | CpaAI (Type II) | R: CP6013_2557 | 52 |
| M: CP6013_2558 |
ND: not detected; NI: not identified.
aMethylated bases in the provided (forward) and reverse strand are underlined and bolded, respectively. Methylation of the CpaAI recognition sequence has not been determined.
bOnly m6A base methylations were detected in this study.