Figure 4. Functional recovery analysis in function of varying wt/mut ratios.

(A) Theoretical model describing activity projections (% pure wt-AQP2) for varying wt/mut ratios (4/0 to 0/4) when assuming a minimal number of functional units within the tetramer to allow activity (n wt-AQP2 = 1 to 4). (B) Transposition of theoretical data, plotting activity levels against wt/mut ratios for each minimal requirement (n = 1 (■), 2 (□), 3 (●) or 4 (○)). Dash line represent non-interacting condition. C) Oocytes were injected with 1 ng mRNA mixtures of varying wt/mut ratios (4/0 to 0/4) combining wt–AQP2 to R254Q, D150E or R187C and incubated for 24 hours prior testing for water permeability. Activities (P_f) are presented in % ± SD of pure wt-AQP2 with n = 8 per condition and is typical of 3 assays. As shown, R254Q display typical dominant negative effect while D150E is compatible with strong functional recovery. As expected, R187C does not affect wt-AQP2 function, in accordance with its non-interfering (monomeric) nature.