Figure 6. Functional recovery of rec-AQP2 forms in mpkCCD_c14 cells.

Cells grown on semipermeable filters were transfected with pBi vectors expressing FLAG-tagged AQP2 variants (V24A, D150E and R187C) as unique transcript (−) or along untagged wt-AQP2 (+) as second transcript (see methods). Immunofluorescence using anti-FLAG allowed visualization of tagged variants to evaluate effective apical membrane targeting. (A) Induction of plasma membrane targeting for V24A and D150E, but not R187C, by coexpression with wt-AQP2. (B) Histogram of positive apical membrane targeting for each condition, presenting mean ± SD of cell count in a 60x field + 8 adjacent fields, for 3 assays. Asterisks indicate statistical significance from -WT condition. (p < 0.001).