Figure 1. Experimental designs to evaluate the effectiveness of HT/PCy extract in animal models.

(A) Mice (n = 6 per group) were bilaterally subjected to surgical DMM or sham operated. HT/PCy (4 g/kg) or GlcN (4 g/kg) were administrated daily by diet supplementation for 12 weeks starting 4 weeks before surgery. A third group was fed with standard diet (control group). Cartilage degradation was evaluated 8 weeks after DMM. (B) Rabbits (n = 6 per group) underwent anterior cruciate ligament transection (ACLT) of the right knee. Vehicle (NaCl 0.9%; negative control), glucosamine (GlcN) (100 mg/kg; positive control) or HT/PCy (100 mg/kg) was orally administrated every two days for 13 weeks starting 3 weeks before surgery. OA severity was evaluated at 10 weeks following surgery by radiographic and histological OARSI scoring.