Figure 2. In vitro HT/PCy effects on iNOS, COX2 and MMP13 expression and NO, PGE₂ and MMP-13 production.

RAC were cultured for 24 h with HT/PCy and treated for an additional 24 h with IL-1β (1 ng/mL; white bar) or without IL-1β (corresponding to 0 ng/mL condition; black bar). The relative expressions of iNOS (A), COX2 (B) and MMP13 (C) compared to β-actin were evaluated by real time RT-PCR. NO production (D) was assessed using Greiss reaction, PGE₂ (E) and MMP-13 (F) productions were measured using ELISA assay. ^#p < 0.05 compared to the condition with IL-1β (1 ng/mL) alone, *p < 0.05 compared to the condition without IL-1β with the same extract solution.