Table 1. Mass spectrometry identification of phenolic metabolites in rabbit serum.
| Compounds | TR (min) | [M–H]− | MS/MS |
|---|---|---|---|
| HT | |||
| hydroxytyrosol glucuronide | 3.0 | 329 | 153 |
| hydroxytyrosol sulfate | 3.1 | 233 | 153 |
| homovanillic acid sulfate | 3.6 | 261 | 181 |
| tyrosol glucuronide | 5.5 | 313 | 137 |
| tyrosol sulfate | 5.7 | 217 | 137 |
| PCy | |||
| catechin glucuronide | 3.5 | 465 | 289 |
| epicatechin glucuronide | 3.8 | 465 | 289 |
| catechin sulfate | 4.0 | 369 | 289 |
| methyl catechin glucuronide | 4.2 | 479 | 303 |
| epicatechin sulfate | 4.4 | 369 | 289 |
| dihydroxyphenylvalerolactone sulfate | 4.5 | 287 | 207 |
| methyl catechin sulfate | 4.6 | 383 | 303 |
| dihydroxyphenylvaleric acid sulfate | 5.1 | 289 | 209 |
| hydroxyphenylpropionic acid | 5.2 | 165 | 121 |
Rabbits received 6 doses of HT/PCy (500 mg/mL) for 8 days, 2 hours after the last dose the serum was harvested. The characterization of the metabolites in the rabbit serum was based on their ion fragmentation in the MS and MS/MS modes. Metabolites were identified by the time retention (TR) (min) and the transition of m/z [M–H]−. Mass spectral characteristic of phenol metabolites in serum from rabbits fed a fraction HT/PCy.